Figure 1.

Deguelin inhibited proliferation of human colorectal cancer cell lines in a dose- and time-dependent manner.

Notes: Cell viability was determined using the CCK-8 assay. (A) SW480, (B) SW620, and (C) RKO cells were treated without or with different concentrations of deguelin for 24 and 48 hours. The IC₅₀ of deguelin for 24 hours in SW480 and SW620 were 122.02 nM and 36.57 nM, respectively. And the IC₅₀ of deguelin for 48 hours in SW480, SW620, and RKO cells were 40.86 nM, 17.73 nM, and 13.25 µM, respectively. (D) SW480 and (E) SW620 cells were cultured with deguelin (100 nM) for different time points. (F) RKO cells were cultured with deguelin (10 µM) for different time points. The data are presented as mean ± SEM for three different experiments performed in triplicate. ^*P<0.05, ^**P<0.01 and ^***P<0.001, compared to control group.

Abbreviations: CCK-8, cell counting kit-8; IC₅₀, 50% inhibitory concentrations; SEM, standard error of the mean.