Table 7.
Normalized levels of proinflammatory cytokines in CD163-overexpressing primary human macrophages
| Cytokines | Experiment | Control | DM2 | DPN | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
| |||||||||||||
| pEmpty | pCD163 | P-value | n | pEmpty | pCD163 | P-value | n | pEmpty | pCD163 | P-value | n | ||
|
| |||||||||||||
| TNF-α | #1 | 1±0.02 | 0.4±0.09 | 0.01 | 2 | 1±0.006 | 1.3±0.4 | 0.39 | 2 | 1±0.2 | 1.05±0.1 | 0.84 | 2 |
| #2 | 1±0.1 | 0.6±0.2 | 0.22 | 2 | 1±0.8 | 0.7±0.7 | 0.76 | 2 | 1±0.4 | 1±0.1 | 0.99 | 4 | |
| #3 | 1±0.4 | 0.4±0.2 | 0.15 | 4 | 1±0.7 | 1.5±1.3 | 0.53 | 4 | 1±0.3 | 0.7±0.5 | 0.46 | 5 | |
| MCP-1 | #1 | 1±0.9 | 0.8±0.5 | 0.83 | 2 | 1±0.3 | 0.6±0.1 | 0.15 | 3 | 1±0.9 | 0.7±0.8 | 0.64 | 6 |
| #2 | 1±0.7 | 0.6±0.8 | 0.48 | 4 | 1±0.1 | 0.8±0.04 | 0.14 | 3 | 1±0.3 | 1±0.3 | 0.86 | 3 | |
| #3 | 1±0.3 | 0.8±0.7 | 0.86 | 2 | 1±0.1 | 0.7±0.03 | 0.14 | 3 | 1±0.5 | 1.6±0.6 | 0.29 | 3 | |
| IL-6 | #1 | 1±1.2 | 0.6±0.7 | 0.74 | 2 | 1±0.6 | 0.2±0.2 | 0.28 | 2 | 1±0.4 | 0.4±0.3 | 0.054 | 5 |
| #2 | 1±0.4 | 0.7±0.1 | 0.50 | 2 | 1±0.1 | 1.2±0.1 | 0.15 | 3 | 1±0.2 | 0.5±0.007 | 0.15 | 2 | |
| #3 | 1±0.1 | 0.2±0.04 | 0.02 | 2 | 1±0.7 | 0.5±0.4 | 0.58 | 2 | 1±0.008 | 0.6±0.8 | 0.02 | 2 | |
| #4 | 1±0.1 | 0.5±0.11 | 0.04 | 2 | |||||||||
| IL-1β | #1 | 1±0.7 | 1.8±0.5 | 0.33 | 2 | 1±0.3 | 1±0.2 | 0.80 | 2 | 1±0.3 | 0.5±0.03 | 0.19 | 2 |
| #2 | 1±0.5 | 1.4±0.7 | 0.56 | 2 | 1±0.1 | 1.1±0.1 | 0.29 | 2 | 1±0.07 | 0.7±0.2 | 0.26 | 2 | |
| #3 | 1±0.5 | 1±0.2 | 0.96 | 2 | 1±0.3 | 1.2±0.006 | 0.42 | 3 | 1±0.8 | 0.9±0.6 | 0.88 | 4 | |
Abbreviations: DM2, diabetes mellitus type 2; DPN, diabetic peripheral neuropathy; TNF-α, tumor necrosis factor-alpha; MCP-1, monocyte chemoattractant protein-1.