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. 2018 Dec 1;34(6):575–579. doi: 10.5423/PPJ.NT.06.2018.0108

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© The Korean Society of Plant Pathology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1 — Primer position within the coat protein of Apple stem grooving virus (ASGV) and the detection of ASGV by RT-RPA. (A) Clustal W multiple sequence alignment was performed with Bio-edit using the sequence of Korea ASGV isolates with isolates from other countries. Unfilled boxes represent the primer regions used in this study. (B) RT-RPA amplification products of ASGV. M, DNA marker; lane 1, ASGV-infected tissues; lane 2, non-infected tissues control. Five independent reactions were performed, and similar results were obtained.