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Sensitivity of RT-RPA. (A) The detection limit of the RT-RPA assay using total RNA isolated from pear leaves infected with ASGV. M, DNA marker; lanes 1–5, serial 10-fold dilutions of RNA (ranging from 47 ng/μl to 4.7 pg/μl). (B) The detection limit of the RT-PCR assay using total RNA isolated from pear leaves infected with ASGV. M, DNA marker; lanes 1–5, serial 10-fold dilution of RNA (ranging from 47 ng/μl to 4.7 pg/μl).
