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Specificity of RT-RPA for ASGV. Total RNAs from the ASGV-, ACLSV-, and ASPV-infected tissues were all tested using RT-RPA. M, DNA marker; lane 1, ASGV-infected tissues; lane 2, ACLSV-infected tissues; lane 3, ASPV-infected tissues; lane 4, virus-free tissues; lane 5, no-template virus. Five independent reactions were performed with similar results observed for all five reactions.
