Table 2.

Comparison of k₂₅ and E_a values for k_cat measurements from the different methods

Method	Temperature (°C)	Parameter	k 25	E a
Radiolabel
Single point	0–25	k catCO2 (s−1)	3.50 ± 0.20 A	79.53 ± 2.03 a
	25–40		–	42.11 ± 3.45 c
Curve fit	10–35		3.10 ± 0.07 A	59.64 ± 3.93 b
MIMS	10–25		3.53 ± 0.25 A	90.36 ± 1.03 a
	25–40		–	62.20 ± 2.68 b
	10–25	k catO2 (s−1)	1.38 ± 0.05 B	92.95 ± 7.31 a
	25–40		–	47.11 ± 2.33 b,c

The k₂₅ and E_a values are the mean of 3–4 replicates, calculated from linear regressions of Arrhenius plots. The temperature ranges for each regression were determined by segment analysis. Letters indicate significant differences between groups (Tukey HSD, P<0.05).