Fig. 10.

DNA methylation status of the CsMYB60 promoter in cucumber fruit with white and black spines. (A) McrBC-sensitive PCR analysis of the promoter of CsMYB60 in RNS8 (white spines) and RNS9 (black spines). + and – indicate whether or not the genomic DNA was treated with McrBC before PCR amplification. (B) McrBC-sensitive PCR analysis of the region from –6468 to about –3006 before the translational start codon (ATG) of CsMYB60 in RNS8 and RNS9. The absence of a PCR product in McrBC-treated samples indicates methylated DNA.