Fig. 8.
Expression of Cs4CL can be activated indirectly by overexpressed CsMYB60 in cucumber cotyledons. (A) GUS staining assays in Nicotiana benthamiana leaves and cucumber cotyledons for proCs4CL::GUS transfection, and pro35S::CsMYB60 and proCs4CL::GUS co-transfection. Scale bars are 0.25 mm. (B) Relative expression of GUS in cucumber cotyledons transfected with proCs4CL::GUS, and pro35S::CsMYB60 plus proCs4CL::GUS. Data are means (±SD) of three technical replicates. The other two biological repeats are shown in Supplementary Fig. S10. Different letters indicate significant differences at P<0.01. Significant differences were determined according to a multiple statistical analysis system using Data Processing System (DPS) statistics version 7.05 software.
