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. 2018 Sep 18;70(1):133–147. doi: 10.1093/jxb/ery334

Fig. 7.

Fig. 7.

Genetic interaction of CIPK25 and SHY2. (A) Relative transcript abundance of SHY2 in 5 dpg Col-0 and cipk25 mutant roots was analyzd by qRT-PCR and semi-quantitative RT-PCR. ACTIN2 was used as the internal control. The right-hand panel shows histochemical GUS staining of primary roots of 5 dpg seedlings expressing the GUS reporter under the SHY2 promoter. The arrow shows the first elongated cortex cell. (B) Primary root length phenotype of 10 dpg Col-0, cipk25, shy2-24, and cipk25 shy2-24 mutants grown vertically on 1/2 MS medium. The right-hand panel shows root length measurement. Error bars represent ±SD, n=24. (C) Relative transcript levels of auxin efflux carriers were analyzed by qRT-PCR in roots of 5 dpg Col-0, cipk25, shy2-24, and cipk25 shy2-24 seedlings. Different letters indicate statistically significant differences by ANOVA, P≤0.05. Error bars are ±SD, n=3. (D) qRT-PCR analysis of the expression levels of CIPK25 in roots of 10 dpg Col-0 and arr1 arr12 seedlings treated with 5 µM t-zeatin for different time intervals. ACTIN2 expression was used as the internal control. Error bars are ±SD, n=3.