Fig. 3.

Some effector fusions accumulate around haustoria. Leaves infected with tdTomato-expressing P. infestans were infiltrated with agrobacteria containing plasmid constructs to express effector fusions transiently. Confocal projection images show cells that are penetrated by haustoria and also expressing effector fusions. Only cells that showed normal subcellular organization were imaged. The left panel shows examples of effector fusions whose main localization was nucleo-cytoplasmic, plasma membrane, and nuclear (from top to bottom) that did accumulate around haustoria. For comparison, the right panel shows effector fusions with the same localizations that did not accumulate around haustoria. The magenta-only panels are included to show the hyphae and haustoria, though many haustoria were either facing the lens or very small and thus cannot be distinguished from the hyphal fluorescence. Haustoria are indicated with arrows. Scale bars represent 10 μm.