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. 2018 Oct 16;70(1):343–356. doi: 10.1093/jxb/ery360

Fig. 6.

Fig. 6.

Virulence tests with combinations with P. infestans RXLR effectors. Transient co-expression of RXLR effectors Pi06087, Pi09585, Pi02860, and Pi11383 with N-terminal GFP tags significantly enhanced P. infestans colonization individually compared with the GFP vector control (A, B). Co-expression of Pi06087 and Pi09585 did not provide an increased effect on P. infestans colonization compared with the effectors alone (A). The co-expression of Pi02860 and Pi11383, however, did provide an increased effect on P. infestans colonization (B). Transient expression of Pi02860 and Pi11383 could suppress cell death triggered by transient co-expression of INF1 or Cf4 and Avr4, respectively (C). Co-expression of the two effectors suppressed cell death triggered by both INF1 and Cf4/Avr4 (C). Boxplots represent the combined data from three biological replicates (n=79 per construct). Letters a, b, and c on the boxplots and graph denote statistically significant differences (ANOVA, P<0.001).