Figure 2. Overview of the cell preparations.

( a) Preparation of Tax ⁺ and Tax ^– populations from PBMCs obtained from HTLV-1-infected patients. PBMCs were stained for CD4, CADM1 and Tax after overnight culture. Tax ⁺ and Tax ^– fractions were collected from the CADM1 ⁺ population. ( b) Preparation of the Tax ⁺ and Tax ^– populations from HTLV-1-infected T cell clones. HTLV-1-infected T cell clones were stained for intracellular Tax and sorted according to Tax expression.