Table 1. Cellular metabolite concentrations from 1D-NMR spectra.

Metabolite concentrations taken from 1D-NMR spectra analysed through Chenomx software.

Metabolite	Cellular Metabolite Concentrations (mM/mg of protein)
	Control		FK866		FK866 NR
	Mean	SEM	Mean	SEM	Mean	SEM
1,3-Dimethylurate	0.00	0.03	0.00	0.01	0.00	0.01
1,7-Dimethylxanthine	0.00	0.00	0.00	0.00	0.00	0.05
2-Aminoadipate	0.01	0.01	0.01	0.01	0.01	0.01
2-Aminobutyrate	0.06	0.08	0.06	0.08	0.07	0.07
2-Hydroxyisobutyrate	0.00	0.00	0.00	0.00	0.00	0.01
2-Hydroxyisocaproate	0.02	0.08	0.04	0.06	0.03	0.05
2-Oxoisocaproate	0.00	0.02	0.00	0.01	0.00	0.01
3-Hydroxyisovalerate	0.00	0.00	0.00	0.00	0.00	0.00
3-Methyl-2-oxovalerate	0.01	0.01	0.01	0.01	0.01	0.01
Acetoacetate	0.01	0.01	0.01	0.02	0.01	0.01
Alanine	0.07	0.12	0.08	0.10	0.09	0.15
Arabinose	0.03	0.05	0.04	0.22	0.04	0.04
Asparagine	0.00	0.01	0.00	0.01	0.01	0.01
Choline	0.01	0.02	0.01	0.01	0.01	0.02
Citrate	0.01	0.02	0.01	0.02	0.02	0.02
Dimethylamine	0.00	0.00	0.00	0.01	0.00	0.00
Fumarate	0.00	0.00	0.00	0.00	0.00	0.00
Galactarate	0.01	0.03	0.02	0.03	0.01	0.03
Glucose	0.00	0.01	0.14	0.01	0.01	0.09
Glutathione	0.05	0.06	0.05	0.06	0.06	0.06
Glycine	0.08	0.18	0.11 *	0.21	0.15	0.20
Hydroxyacetone	0.01	0.02	0.01	0.02	0.02	0.02
Imidazole	0.00	0.02	0.01	0.00	0.00	0.01
Nicotinamide N-oxide	0.00	0.00	0.00	0.00	0.00	0.00
O-Acetylcarnitine	0.00	0.01	0.00	0.01	0.00	0.01
O-Acetylcholine	0.01	0.02	0.01	0.02	0.01	0.01
Phenylalanine	0.04	0.12	0.05	0.10	0.03	0.09
Pyruvate	0.01	0.01	0.01	0.02	0.01	0.02
Succinate	0.00	0.01	0.01	0.01	0.01	0.01
Taurine	0.26	0.45	0.29 *	0.47	0.22	0.51
Threonine	0.07	0.09	0.09 *	0.24	0.07	0.09
Tryptophan	0.01	0.02	0.01	0.01	0.01	0.02
Tyrosine	0.05	0.16	0.08	0.14	0.04	0.12
Valine	0.06	0.20	0.10	0.18	0.06	0.16

* p<0.05; Two-way ANOVA performed on all data; Dunnett’s multiple comparison test. n=6.