Table 2. Cellular metabolite ion counts from GCMS.
Ion counts taken from GCMS. No significant changes seen between conditions.
| Metabolite | Cellular metabolite ion counts | |||||||
|---|---|---|---|---|---|---|---|---|
| Control | FK866 24h | FK866 48 h | FK866 48 h, NR 4h | |||||
| Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | |
| Tryptophan | 49720 | 57190 | 64405 | 55845 | 53202 | 52914 | 55327 | 47893 |
| Serine | 415367 | 164737 | 237489 | 187173 | 845253 | 783737 | 686931 | 1055501 |
| Pyruvate | 255065 | 168133 | 146860 | 97016 | 124497 | 13889 | 358317 | 191197 |
| Proline | 163717 | 121858 | 231244 | 177360 | 174065 | 207804 | 15691 | 11678 |
| Malate | 147186 | 112783 | 114083 | 89299 | 73951 | 64911 | 142479 | 159432 |
| Lactate | 8345878 | 7825695 | 8367333 | 5174489 | 7446052 | 5382583 | 8998869 | 3275040 |
| Histidine | 646757 | 551284 | 741234 | 488925 | 706205 | 654021 | 605624 | 362069 |
| Glycine | 9116697 | 7104045 | 8635419 | 6465350 | 7972076 | 7955743 | 8503790 | 6814356 |
| G3P | 1174906 | 826308 | 1530734 | 1046045 | 2273705 | 2037600 | 1700887 | 1223394 |
| Glutamine | 942572 | 1003918 | 1448528 | 791723 | 1545368 | 1797213 | 898866 | 858392 |
| Fumarate | 81303 | 67667 | 90462 | 60750 | 91008 | 76830 | 95761 | 69124 |
| Cysteine | 203170 | 263833 | 356747 | 119714 | 369724 | 210067 | 311876 | 86770 |
| Alanine | 1541322 | 730418 | 967774 | 739846 | 1398345 | 1367006 | 1349550 | 1382444 |
| 3-PG | 11227 | 11048 | 15149 | 6286 | 22499 | 33977 | 16770 | 11633 |
*** p<0.005; Two-way ANOVA performed on all data; Dunnett’s multiple comparison test. n=4.