Fig. 2.

—Strain development for genome sequencing (steps 1–13), BSA (steps 14–18), and developmental transcriptomics (steps 19–20). Mating alleles of single-spore isolates derived from the parental and tester dikaryons were arbitrarily numbered A1, B1 (RLG-9953), A3, B3 (DAOM 54158), and A5, B5 (FP 102501-T). Mating type alleles of single-spore progeny were not determined after step 2. Accordingly, mating types are designated An, An*, Bn, and Bn*, where An ≠ An* and Bn ≠ Bn*. Because Lentinus tigrinus is heterothallic and tetrapolar, all dikaryons produced after step 2 must be A1, B1/A3, B3. See text for further details.