Table 1.

Standard primer pairs used in this study.

Target genes	Sequence (5′→3′)	Amplicon size(bp)	Reference
mcr-1	CGGTCAGTCCGTTTGTTC	350	Liu et al., 2016
	CTTGGTCGGTCTGTA GGG
mcr-2	TGGTACAGCCCCTTTATT	1617	Xavier et al., 2016
	GCTTGAGATTGGGTTATGA
mcr-3	TTGGCACTGTATTTTGCATTT	542	Yin et al., 2017
	TTAACGAAATTGGCTGGAACA
mcr-4	ATTGGGATAGTCGCCTTTTT	487	Carattoli et al., 2017
	TTACAGCCAGAATCATTATCA
mcr-5	ATGCGGTTGTCTGCATTTATC	1644	Borowiak et al., 2017
	TCATTGTGGTTGTCCTTTTCTG
bla KPC	ATGTCACTGTATCGCCGTC	902	Zheng et al., 2015
	TTACTGCCCGTTGACGCC
blaOXA-48	TTGGTGGCATCGATTATCGG	744	Zheng et al., 2015
	GAGCACTTCTTTTGTGATGGC
bla NDM	ATGGAATTGCCCAATATTATGCAC	813	Zheng et al., 2015
	TCAGCGCAGCTTGTCGGC
blaV IM	TTTGGTCGCATATCGCAACG	500	Zheng et al., 2015
	CCATTCAGCCAGATCGGCAT
bla IMP	GTTTATGTTCATACWTCG	432	Zheng et al., 2015
	GGTTTAAYAAAACAACCAC
blaSHV	ATTTGTCGCTTCTTTACTCGC	861	Zheng et al., 2015
	TTTATGGCGTTACCTTTGACC
bla TEM	ATGAGTATTCAACATTTCCGTG	861	Zheng et al., 2015
	TTACCAATGCTTAATCAGTGAG
blaCTX-M	TTTGCGATGTGCAGTACCAGTAA	759	Zheng et al., 2015
	CGATATCGTTGGTGGTGCCATA
bla CTX-M-1	AAAAATCACTGCGCCAGTTC	415	Zheng et al., 2015
	AGCTTATTCATCGCCACGTT
bla CTX-M-9	CAAAGAGAGTGCAACGGATG	205	Zheng et al., 2015
	ATTGGAAAGCGTTCATCACC
fosA3	GCGTCAAGCCTGGCATTT	282	Hou et al., 2012
	GCCGTCAGGGTCGAGAAA
qnrB	GATCGTGAAAGCCAGAAAGG	469	Wang et al., 2017
	ACGATGCCTGGTAGTTGTCC
qnrS	ACGACATTCGTCAACTGCAA	540	Wang et al., 2017
	TAAATTGGCACCCTGTAGGC
oqxAB	CCCTGGACCGCACATAAAG	1140	Wang et al., 2017
	AAAGAACAAGATTCACCGCAAC
sul1	ATGGTGACGGTGTTCGGCATTCTG	840	Hur et al., 2011
	CTAGGCATGATCTAACCCTCGGTC
sul2	GAATAAATCGCTCATCATTTTCGG	810	Hur et al., 2011
	CGAATTCTTGCGGTTTCTTTCAGC
tetM	AGTGGAGCGATTACAGAA	158	Adefisoye and Okoh, 2016
	CATATGTCCTGGCGTGTCTA
tetA	GCTACATCCTGCTTGCCTTC	210	Adefisoye and Okoh, 2016
	CATAGATCGCCGTGAAGAGG
aac(6′)-Ib-cr	TTGCGATGCTCTATGAGTGGCTA	482	Eftekhar and Seyedpour, 2015
	CTCGAATGCCTGGCGTGTTT
rmtA	CTAGCGTCCATCCTTTCCTC	635	Wang et al., 2017
	TTGCTTCCATGCCCTTGCC
rmtB	GCTTTCTGCGGGCGATGTAA	173	Wang et al., 2017
	ATGCAATGCCGCGCTCGTAT
floR	GTCGAGAAATCCCATGAGTTCA	1645	Cloeckaert et al., 2000
	CAGACAGGATACCGACATTCAC
intI1	GGGTCAAGGATCTGGATTTCG	484	Mazel et al., 2000
	ACATGCGTGTAAATCATCGTCG
vanA	AATACTGTTTGGGGGTTGCTC	734	Kafil and Asgharzadeh, 2014
	TTTTTCCGGCTCGACTTCCT
mecA	TGGTATGTGGAAGTTAGATTGGGAT	155	Paterson et al., 2012
	CTAATCTCATATGTGTTCCTGTATTGGC
ermB	GATACCGTTTACGAAATTGG	364	Zhang et al., 2016
	GAATCGAGACTTGAGTGTGC
ermF	CGACACAGCTTTGGTTGAAC	309	Zhang et al., 2016
	GGACCTACCTCATAGACAAG
aph(3′)-IIIa	GCC GAT GTG GAT TGC GAA AA	269	Udo and Dashti, 2000
	GCT TGA TCC CCA GTA AGT CA