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. 2018 Dec 18;9:633. doi: 10.3389/fgene.2018.00633

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Copyright © 2018 Li, Struebing, Wang, King and Geisert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Whole mount retinal immunohistochemistry showing AAV transduction in RGCs. (A) Confocal image of whole mounted retina showing transduction of AAV2-CMV-GFP. (B) Confocal image of whole mounted retina showing transduction of AAV2-shRNA- Pten-GFP. (C) Confocal image of flat mounted retina showing GFP expressing in RGCs. (D) RBPMS label RGCs specifically as a marker. (E) More than half of RGCs are transduced by AAV and express GFP; merge image. Scale bar = 100 μm.