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. 2018 Dec 18;9:633. doi: 10.3389/fgene.2018.00633

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Copyright © 2018 Li, Struebing, Wang, King and Geisert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Downregulation of Sox11 facilitates RGC survival following optic nerve crush and regeneration experiment. (A) Sox11 and Pten are knocked down by intravitreal injection of AAV2-CMV-Cre-GFP or AAV2-shRNA-Pten-GFP in 4 to 6 week-old Sox11^f/f mice. The control group receives AAV2-CMV-GFP. Optic nerve crush is performed 2 weeks after viral vector injection. Twelve days after ONC, Alexa Fluor^® 647-conjugated CTB is used to label regenerating axons. Two days after the CTB injections the animals are sacrificed, the retinas and full-length optic nerves are removed. (B) RGCs are labeled with pan-RGC marker RBPMS in retinal whole mounts. (C) Downregulation of both Sox11 and Pten resulted in a significant increase in RGC survival as compared to Pten knockdown only (1393 ± 94/mm² vs. 722 ± 77/mm², n = 4, ^∗p < 0.05, Mann–Whitney U-test). Scale bar = 100 μm.