Table 1.

Comparison of single locus sequencing results to whole genome sequencing results for sixteen MAC strains for each sample that was whole genome sequenced, three loci were extracted and analyzed by BLAST to the non-redundant NCBI database giving the results listed.

	Region extracted from WGS data
Sample	16S (1026bp)	16S 403∗	ITS (335-336bp)	rpoB (752bp)	MBT subtyper	WGS
NTM-006	M. chim/M.int	M. int	M. chim/M.int	M. chim	M. int	M. int
NTM-168	MAC	M. int	M. chim/M.int	M. int	M. chim/M.int	M. int
NTM-224	M. mars/M.int	M. chim	M. int	M. chim	M. chim/M.int	M. chim
NTM-105	MAC	M. int	M. chim/M.int	M. chim	M. int	M. chim
NTM-206	MAC	M. int	M. chim/M.int	M. chim	M. int	M. chim
NTM-035	MAC	M. int	missing data	M. chim	M. int	M. chim
NTM-232	MAC	M. int	M. chim/M.int	M. chim	M. int	M. chim
NTM-019	M. chim/M.int	M. int	M. chim/M.int	M. chim	M. int	M. chim
NTM-178	MAC	M. int	M. chim/M.int	M. chim	M. int	M. chim
NTM-107	MAC	M. int	M. chim/M.int	M. chim	M. int	M. chim
NTM-054	M. mars/M.int	M. chim	M. int/M.int yong	M. chim	M. int	M. chim
NTM-223	MAC	M. int	M. int	M. chim	M. chim/M.int	M. chim
NTM-203	MAC	M. int	M. int	M. chim	M. chim/M.int	M. chim
NTM-208	MAC	M. int	M. int	M. chim	M. chim/M.int	M. chim
NTM-204	MAC	M. int	M. int	M. chim	M. chim/M.int	M. chim
NTM-230	M. chim	M. chim	M. chim	M. chim	M. chim/M.int	M. chim

Also listed are the species as determined using only the 403 SNP from 16S, assuming a strain was previously identified as belonging to the chimaera/intracellulare group. MBT Subtyper result and the WGS species call based on phylogenomic tree. M. chim = M. chimaera, M. int = M. intracellulare, M. yong = M. yongonense, M. mars = M. marseillense.