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. 2018 Dec 18;9:2972. doi: 10.3389/fimmu.2018.02972

Figure 1.

Figure 1

Pathological changes in skin of trout following Ich parasites infection. (A) Histological examination (hematoxylin/eosin stain) of skin from trout infected with Ich after 12, 24, 48, 72 h, 7, 21, 28 d and control fish (n = 6 fish per group). (B) Immunofluorescence staining for Ich (green) in skin paraffin sections from trout infected with Ich after 7 d is shown in the left, and the right panel display staining of trout skin with isotype control antibodies for anti-Ich. Differential interference contrast images (DIC) of skin are stained with the DNA-intercalating dye DAPI (blue) (n = 6 fish per group). (C) The thickness of skin epidermis of control and infected fish (n = 6 fish per group). (D) The numbers of mucus cells per millimeter in skin tissue of control and infected fish (n = 6 fish per group). (E) The expression of Ich-18SrRNA in trout skin was detected by qRT-PCR. The data was shown as –ΔΔCt. Skin structure is always displayed with the outside part of the epidermis (Ep) and the dermis (De). Scales (Sc), and melanophores (Me) are also indicated with black letters. The black line with double arrowheads indicates thickness of skin epidermis. Scale bars, 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001 (unpaired Student's t-test). Data are representative of three different independent experiments (mean ± s.e.m.).