Figure 10.

E. faecium + Inulin supplementation reverts the loss of synaptic potentiation induced with Tetraethylammonium (TEA). (A) Averaged time course graph contrasting the lack of synaptic potentiation of control slices (gray symbols; n = 8 cells/5 animals for control condition and 8 cells/5 animals for the symbiotic group) contrasted with the responses obtained from supplemented animals (empty symbols; n = 10 cells/6 animals for control condition and 10 cells/6 animals for the symbiotic group). TEA (20 mM) was bath perfused for 20 min (indicated with the filled bar) and then washed out. TEA stimulation resulted in a sustained increase in the fEPSP slope in the E. faecium + Inulin supplemented slices but not in control slices. (B) Box plots summarizing fEPSP changes at 5 min of TEA perfusion and 60 min post-TEA in control (gray boxes) and E. faecium + Inulin supplemented group (empty boxes). The symbols on left side of the boxes represent the average response from each experiment. *p < 0.05, **p < 0.01. (C) Representative extracellular CA1 responses (averaged from 10 consecutive sweeps; recorded in the CA1 stratum radiatum) obtained at the times indicated by the numbers in the time-course graph in upper panel (A), of control (top traces) and supplemented animals (bottom traces).