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. 2018 Dec 18;9:1866. doi: 10.3389/fpls.2018.01866

FIGURE 1.

FIGURE 1

Analysis of the interaction between JAZ and DELLA proteins in rice. (A) A Y2H assay showing the interaction between OsJAZs and SLR1. Co-transformation with the p53 bait and T prey plasmid was used for a positive control (P) and co-transformation with the LAM bait and T prey plasmid was used for a negative control (N). -Leu/-Trp/+Abs A indicates aureobasidin A-containing DDO media to test for the JAZs-DELLA interaction. -Leu/-Trp means DDO media to verify yeast transformation of the indicated bait and prey plasmid. J1–J12 indicates the OsJAZ1–OsJAZ12 bait plasmids used for yeast transformation. Blank indicates empty bait or prey plasmid. (B) Co-IP results showing the interaction between OsJAZ9 and SLR1. Total proteins were extracted from rice protoplasts co-transformed with 6xMyc-OsJAZ9 and GFP-OsSLR1. IP indicates immunoprecipitation, and α-GFP (left) and α-Myc (right) indicate the antibodies used for the immunoprecipitation. α-Myc (left) and α-GFP (right) are the antibodies used for western blotting to detect the interaction between OsJAZ9 and SLR1. (C) A bimolecular fluorescence complementation (BiFC) assay showing the interaction between OsJAZ9 and SLR1 protein. Onion epidermal cells were co-transfected with nYFP-SLR1 and OsJAZ9-cYFP (top), nYFP-SLR1 and empty cYFP (middle), or empty nYFP and OsJAZ9-cYFP (bottom) plasmids by tungsten particle bombardment. The yellow channel image is shown in the YFP panel and the bright-field panel shows the differential interference contrast image. Scale bars = 50 μm.