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. 2018 Dec 18;9:1866. doi: 10.3389/fpls.2018.01866

FIGURE 2.

FIGURE 2

Identification of the domains in OsJAZ9 and SLR1 proteins that mediate their interaction. (A) Schematic of the truncated OsJAZ9 proteins used to analyze the interaction with DELLA proteins. The colored boxes indicate the ZIM (yellow) and Jas (green) domains (Seo et al., 2011). (B,C) Truncated OsJAZ9 bait constructs were co-transformed with SLR1 (B) or Arabidopsis RGA (At2G01570) (C) prey constructs and plated out on DDO media containing Abs A. -Leu/-Trp/+Abs A and -Leu/-Trp indicate DDO media with and without aureobasidin A, respectively. (D) Schematic of the truncated SLR1 proteins used to test the interaction with JAZ proteins. The colored boxes indicate the DELLA (orange) and GRAS (blue) domains (Ueguchi-Tanaka et al., 2007; Hirano et al., 2010). (E,F) Yeast lines were co-transformed with OsJAZ bait plasmids (J1–J12) together with truncated SLR1D (E) or SLR1G (F) prey plasmids and plated on Abs-A containing media.