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IFNγ production of alloreactive CD4 T-cell clones. Recognition of patient and donor derived EBV-LCL by CD4 T-cells measured by INFγ production after overnight incubation. Each dot represents the INFγ release by one alloreactive CD4 T-cell clone after overnight incubation in a responder to stimulator ratio of 1:10. All CD4 T-cell clones did recognize patient derived EBV-LCL and did not recognize donor derived EBV-LCL and are therefore defined as alloreactive.
