Table 3.

Classification of alloreactive T-cell clones according to HLA restriction molecule and TCR Vβ usage.

Patient	HLA class II restriction molecule#	TCR Vβ usage	Identified MiHA	Number of clones
Patient A	undetermined	unknown	–	1*
	DQ	2	LB-LILRB1-1I	6*
	DQ	2	–	2*
	DQ	9	–	1*
Patient B	DR	8	LB-ABCA5-1R	1*
	DR	13.2	LB-RPS4Y	1*
Patient C	DR	2	SCL19A1	21*
	DR	5.3	SCL19A1	1‡
	DR	unknown	LB-ZDHHC13-1K	10*
	DR	unknown	LB-KHNYN-1K	3*
	DR	7.1	LB-KHNYN-1K	1‡
	DR	9	LB-CTSB-1G	1*
	DR	13.2	LB-CTSB-1G	1‡
	DR	9	LB-LGALS8-1C	2*
	DR	unknown	LB-LGALS8-1C	1*
Patient D	undetermined	3	–	2*
	DQ	5.1	–	1
	DP	2	LB-LY75-2R	38*
	DP	3	LB-LY75-2R	1‡
	DP	4	LB-LY75-2R	32‡
	DP	8	LB-LY75-2R	6‡
	DP	13.2	LB-LY75-2R	3‡
	DP	unknown	LB-LY75-2R	27*

^#HLA class II restriction molecule was determined using monoclonal antibodies against pan HLA class II (PdV5.2), HLA-DR (B8.11.2), HLA-DQ (SPVL3) and HLA-DP (B7.21).

^*WGAS was performed using one of these T-cell clones.

^‡MiHA specificity of these T-cell clones was determined by recognition of donor EBV-LCL loaded with the MiHA identified using WGAS analysis of another T-cell clone.