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. 2018 Dec 18;9:3023. doi: 10.3389/fimmu.2018.03023

Figure 3.

Figure 3

TRAIP interacts with PRRSV-nsp1α. (A) HEK293 cells were first transfected with Flag-TRAIP and then co-transfected with Myc-nsp1, Myc-nsp1α, Myc-nsp1β, vector expressing plasmids. The cell lysates were then immunoprecipitated with an anti-Myc MAb and detected by Western blotting at 24 h post-transfection. (B,C) Co-localization of nsp1α protein with TRAIP in the cytoplasm and nucleus in HeLa cells and 3D4/21 cells. HeLa or 3D4/21 cells were seeded in 12-well plates and co-transfected with Flag-TRAIP and Myc-nsp1α expressing plasmid. At 18 h post-transfection, the cells were incubated with a rabbit anti-Myc mAb and a mouse anti-Flag antibody followed by FITC-conjugated anti-mouse IgG (green) and PE-conjugated anti-rabbit IgG (red) or FITC-conjugated anti-rabbit IgG (green) and PE-conjugated anti-mouse IgG (red). (D) Porcine TRAIP co-localize with PRRSV-nsp1α in PRRSV infection of 3D4/21 cells. Nuclei were stained with DAPI (blue). Cells were observed under a laser confocal imaging analysis system, scale bar: 7 μm.