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. 2018 Dec 18;9:3023. doi: 10.3389/fimmu.2018.03023

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Copyright © 2018 Shi, Su, Li, Zhang, Chen, Zhang, Faaberg and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nsp1α removed SUMO-modification of TRAIP. HEK 293T cells were transfected with HA-nsp1α and Flag-TRAIP, and lyzed in RIPA buffer containing desumoylation protease inhibitor N-Ethylmaleimide [NEM] inhibition of deSUMOylation of TRAIP. The effect of nsp1α on TRAIP SUMO modification was detected using SUMO1 antibody (A) or SUMO2/3 antibody (B). (C) HEK 293T cells were co-transfected with HA-nsp1α and Flag-TRAIP, and the effect of nsp1α on exogenous TRAIP SUMO-modification was detected by Western blotting. (D) HEK 293T cells were transfected with HA-nsp1α or vector and the effect of nsp1α on endogenous TRAIP SUMO modification was detected by WB. HEK 293T cells were cotransfected with Myc-nsp1α and Flag-TRAIP (E,F) and the effect of nsp1α on endogenous TRAIP SUMO modification was detected by co-immunoprecipitation.