Table 1.
Primers used for PCR amplification.
| Primer name | Genbank number | Sequence of primer(5′-3′) |
|---|---|---|
| T-TRAIP-F | XM_021068793.1 | GAGACCAGTCATGCCTATTCG |
| T-TRAIP-R | TGTTTTCACTAGGACAGGAAAC | |
| pCMV-TRAIP-F | XM_021068793.1 | ATTCGAATTTAAATCGGATCCATGCCTATTCGTGCTCTG |
| pCMV-TRAIP-R | ATCCTTCGCGGCCGCGGATCCCTAGGACAGGAAACTGT | |
| pet-28a-TRAIP-F | XM_021068793.1 | ACAGCAAATGGGTCGCGGATCCATGTATCGTGCAGCGGAT |
| pet-28a-TRAIP-R | GACGGAGCTCGAATTCGGATCCCAGACTTGTCTCCTCA | |
| pCMV-TRAIP(1-177)-F | XM_021068793.1 | CCAGTCGACTCTAGAGGATCCATGCCTATTCGTGCTCTGTGC |
| pCMV-TRAIP(1-177)-R | CAGGGATGCCACCCGGGATCCCTACCGCTGGCTCTGGAGTAGG | |
| pCMV-TRAIP(1-395)-F | XM_021068793.1 | CCAGTCGACTCTAGAGGATCCATGCCTATTCGTGCTCTGTGC |
| pCMV-TRAIP(1-395)-R | CAGGGATGCCACCCGGGATCCCTACCGGATGAAAACAGGGAAG | |
| pCMV-TRAIP(54-472)-F | XM_021068793.1 | ATTCGAATTTAAATCGGATCCATGCAAAAGAACCATTATCA |
| pCMV-TRAIP(54-472)-R | ATCCTTCGCGGCCGCGGATCCCTAGGACAGGAAACTGTCCAGC | |
| pCMV-TRAIP(178-472)-F | XM_021068793.1 | CCAGTCGACTCTAGAGGATCCATGCCTGAGGTGGAGGAAATGAT |
| pCMV-TRAIP(178-472)-R | CAGGGATGCCACCCGGGATCCCTAGGACAGGAAACTGTCCAGCT | |
| pEGFP-TRAIP-F | XM_021068793.1 | GTACCGCGGGCCCGGGATCCATGCCTATTCGTGCTCTG |
| pEGFP-TRAIP-R | TTATCTAGATCCGGTGGATCCGGACAGGAAACTGTCC | |
| pCMV-nsp1-F | MF187956.1 | CCAGTCGACTCTAGAGGATCCATGTCTGGGATACTTGATCGGTG |
| pCMV-nsp1- R | CAGGGATGCCACCCGGGATCCACCGTACCACTTATGACTGCCAA | |
| pCMV-nsp1α-F | MF187956.1 | CCAGTCGACTCTAGAGGATCCATGTCTGGGATACTTGATCGGTG |
| pCMV-nsp1α-R | CAGGGATGCCACCCGGGATCCCTACATAGCACACTCAAAAGGGC | |
| pCMV-nsp1β-F | MF187956.1 | CCAGTCGACTCTAGAGGATCCATGGCTGACGTCTATGACATTGGT |
| pCMV-nsp1β-R | CAGGGATGCCACCCGGGATCCCTAACCGTACCACTTATGACTGC | |
| pCMV-nsp1α N(1-167)-F | MF187956.1 | CCAGTCGACTCTAGAGGATCCATGTCTGGGATACTTGATCGGTG |
| pCMV-nsp1α N(1-167)-R | CAGGGATGCCACCCGGGATCCCTACCTCTGCGGGAGCGGCAA | |
| pCMV-nsp1α N(67-180)-F | MF187956.1 | CCAGTCGACTCTAGAGGATCCATGACTGTCGAGTGCTCCCCCG |
| pCMV-nsp1α N(67-180)-R | CAGGGATGCCACCCGGGATCCCTACATAGCACACTCAAAAGGGC |