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. 2018 Dec 18;11:451. doi: 10.3389/fnmol.2018.00451

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Copyright © 2018 Wang, Yuan, Zhang, Wang, Liu, Song, Zhong, Chen and Dong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of peripheral and central Angptl8 on BAT mass, BAT morphology, and Ucp-1 expression. Angptl8 (30 μg/ml) via I.V. injection (n = 6 and 3 in experimental and control group, respectively) decreased BAT deposit (A) but had no effect on BAT cell density (B) and Ucp-1 expression (C–E). (D) Use β-actin as the HKG and (E) use GAPDH as the HKG. Angptl8 (0.3 μg/μl) via I.C.V. injection (n = 7 and 3 in experimental and control group, respectively) increased BAT deposit (F) but had no effect on BAT morphology (G) or Ucp-1 expression (H–J). (I) Use β-actin as the HKG and (J) use GAPDH as the HKG. Data are means ± SEM. ^∗P < 0.05 relative to the NS control group.