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. 2018 Dec 24;8:18062. doi: 10.1038/s41598-018-36138-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of the effect of MLC901 treatment in inflammatory marker gene expression in mouse brains after MCAO. Arrays of interleukin, cytokine (A,B, left panels) and CC chemokine ligand and receptor (A, B, right panels) gene expression in the injuried cortex of vehicle- and MLC901-treated mice 24 hours after MCAO with (A,C) or without reperfusion (B,D) (n = 6 per experimental group). MCAO-induced fold upregulation versus sham group was calculated using the ∆∆C_T method according to the manufacturer protocol. Data are expressed as mean ± SEM. ^#P < 0.05, ^##P < 0.01 versus sham and ^$P < 0.05 versus vehicle-group (n = 5 per experimental group) (Mann & Whitney test).