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. 2018 Dec 24;8:18076. doi: 10.1038/s41598-018-36197-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Temporal profiling of glycolytic activity during the 48 h intraerythrocytic development of P. falciparum. (a) Giemsa stained smears showing the different developmental stages of P. falciparum at 6 h intervals following sorbitol synchronization of the parasites. U¹³C-glucose metabolism (b) and U¹³C-lactate production (c) by ring stage (culture sampled from 0.5 hps to 6 hps) and late trophozoite stage (culture sampled from 30 hps to 36 hps) parasites respectively. Data was plotted as in Fig. 1. Glycolytic profile of iRBCs during the complete 48 h intraerythrocytic developmental cycle is plotted as either the rate of glucose depletion (d) or rate of lactate formation (e), respectively.