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. 2018 Dec 24;8:18059. doi: 10.1038/s41598-018-36345-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Species distribution in the three size-fractions and from light microscopy (non-fractionated) samples from Naples subsurface (ss) and DCM waters (October 2009) and Oslo subsurface waters (September 2009). rDNA and rRNA results were merged. Data from Oslo ’09 are from Dittami et al.⁵⁸. The main taxa detected through metabarcoding matched those seen by light microscopy of the corresponding samples. The relative abundance of taxa from cell counts was generally more similar to that detected by sequences in the nanoplanktonic size fraction.