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. 2018 Dec 18;9:2928. doi: 10.3389/fimmu.2018.02928

Figure 5.

Figure 5

Impaired Th2 immunity in mice lacking IL-4 producing B cells. Bone marrow chimeras were infected with 100 live S. mansoni cercariae and killed 7 weeks post-infection. Single cell suspensions were prepared from MLN and cells were restimulated with 20 μg/ml SEA or α-CD3 in vitro. (A,B) Cytokine production by restimulated total MLN cells was detected by ELISA. (C) Frequency of CD19+B220+ B cells in the gut draining lymph node. (D,E) Detection of intracellular cytokines produced by CD19+ B cells after restimulation of total MLN cells with 50 ng/ml PMA and 250 ng/ml ionomycin. (F) Frequency of CD3+CD4+ T cells in the MLN. (G,H) Intracellular cytokine production by CD3+CD4+ T cells after stimulation of total MLN cells with 50 ng/ml PMA and 250 ng/ml ionomycin. (I) Frequency of CD3+CD4+ T cells expressing Gata-3. (J) Dot plot showing gating on IL-4 producing CD4+ T cells by infected WT, B-IL-4−/− and IL-4−/− chimeras. Data are representative of two independent experiments. n = 4–6 mice.