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. 2018 Dec 18;9:1810. doi: 10.3389/fphys.2018.01810

FIGURE 2.

FIGURE 2

AII has no effect on expression of integrin α5 in VSMCs. Representative images for six cells studied under each condition. Note should be made that it cannot be excluded that some of the cells were non-responders as these cells were not subjected to AFM functional studies for technical reasons. (A–D) Immunostaining of integrin α5 (red) and labeling with phalloidin (green) on VSMCs before treatment (A), after addition of AII (10-6 M) for 30 min (B) and PBS (C). (D) Is negative control without anti-integrin α5 as the primary antibody. Scale bars represent 10 μm.