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. 2018 Dec 18;9:3117. doi: 10.3389/fmicb.2018.03117

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Copyright © 2018 Jing, Cao, Zhang, Cheng, Gilbert, Dai, Sun, Liang and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The activation of silent var gene by its artificial aslncRNA. The expression of var family and artificial aslncRNA were detected in the transformant C8/pT7SE-as0617400 (A,B, respectively) and the negative control C8/pT7SE-as0617400 (C,D, respectively). PF3D7_0617400 was distinct expressed in C8/pT7SE-as0617400 but not C8/pT7-as0617400. ^∗P < 0.05 by paired two-tailed Student’s t-test. (A–D) Relative transcripts numbers are normalized to serine-tRNA ligase gene (PF3D7_0717700). The RT-qPCR results are representative of three independent experiments with data indicating the mean+SD.