Skip to main content
. 2018 Dec 18;9:3117. doi: 10.3389/fmicb.2018.03117

FIGURE 3.

FIGURE 3

Silent var gene could be activated by its artificial aslncRNA lacking the intron sequence. (A) The schematic diagram of the episome for expressing the artificial PF3D7_0617400 aslncRNA lacking intron sequence. This plasmid was then used to transfected into C8 strain. Specific primer pairs p1/p2 and p3/p4 were used for the exogenous and endogenous artificial aslncRNA detection, respectively. T7 pro, T7 promoter; T7 ter, T7 terminator. (B) The expression of the exogenous and endogenous PF3D7_0617400 aslncRNAs in C8/pT7SE-as0617400-exonI (black) and C8 strain (gray). (C) The var gene expression pattern in C8/pT7SE-as0617400-exonI. In this transformant, the artificial PF3D7_0617400 aslncRNA was lacking intron region. PF3D7_0617400 was distinct expressed in C8/pT7SE-as0617400-exonI but not C8/pT7-as0617400. (B,C, respectively) Relative transcripts numbers are normalized to serine-tRNA ligase gene (PF3D7_0717700). The RT-qPCR results are representative of three independent experiments with data indicating the mean+SD. ∗P < 0.05 by paired two-tailed Student’s t-test.