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. 2018 Dec 18;9:2999. doi: 10.3389/fimmu.2018.02999

Figure 1.

Figure 1

Involvement of host miR-1000 in virus infection. (A) Downregulation of miR-1000 in shrimp in response to virus infection. Shrimp were challenged with WSSV and then subjected to Northern blot analysis to detect miR-1000 expression level. U6 was used as a control. Numbers indicated the time points post-infection. (B) Overexpression of miR-1000 in shrimp. Shrimp were injected with miR-1000 and WSSV. At different time points post-infection, the expression level of miR-1000 was evaluated using Northern blot. (C) Effects of miR-1000 overexpression on WSSV infection. Shrimp were co-injected with miR-1000 or miR-1000-scrambled and WSSV, followed by the detection of WSSV copies. WSSV alone was used as a positive control. (D) Cumulative mortality of shrimp. PBS alone, miR-1000 alone and non-treated shrimp were used as negative controls. (E) Silencing of miR-1000 in shrimp. Shrimp were co-injected with AMO-miR-1000 and WSSV. At different time points post-infection, the expression level of miR-1000 was evaluated using Northern blot. (F) Influence of miR-1000 silencing on virus infection. (G) Cumulative mortality of shrimp. As negative controls, non-treated shrimp and shrimp injected with PBS or miR-1000 were assayed. Data presented here were the mean ± standard deviation of three independent experiments. Significant differences are indicated by asterisks (*p < 0.05; **p < 0.01).