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. 2018 Dec 18;9:2999. doi: 10.3389/fimmu.2018.02999

Figure 3.

Figure 3

Effects of wsv191 and wsv407 gene silencing on WSSV infection. (A) Northern blot analysis of wsv191, wsv407, and wsv024 gene expressions in WSSV-infected shrimp. Numbers indicated the time post-infection. (B) Silencing of wsv191 and wsv407 gene expressions in shrimp. Shrimp were infected with WSSV. At 48 h post-infection, shrimp were injected with wsv191-siRNA or wsv407-siRNA. Twelve hours later, shrimp were re-injected with the siRNA. At different time points after the last siRNA injection, the mRNA levels of wsv191 and wsv407 were determined using Northern blot. Numbers represented the time after the last siRNA injection of WSSV-infected shrimp. (C) Western blots showing the silencing of wsv191 and wsv407 in shrimp in vivo. (D) Influence of wsv191 and wsv407 gene silencing on WSSV virus infection in shrimp. Shrimp hemocytes were subjected to quantitative real-time PCR to determine WSSV copies. (E) Effects of wsv191 and wsv407 silencing on shrimp mortality. The mortality of shrimp per different treatment groups was examined daily. The treatments were shown on the top. Significant statistical differences between treatments were indicated with asterisks (*p < 0.05; **p < 0.01).