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. 2018 Dec 18;9:2999. doi: 10.3389/fimmu.2018.02999

Figure 4.

Figure 4

Suppression of WSSV infection by miR-1000 via targeting wsv191 and wsv407 in vivo. (A) Northern blot analysis of wsv191 and wsv407 mRNA levels in shrimp. Shrimp were injected with WSSV, AMO-miR-1000, wsv191-siRNA and/or wsv407-siRNA. Forty 8 h later, shrimp hemocytes were subjected to Northern blot. (B) Western blot analysis of wsv191 and wsv407 protein levels. At 48 h after injection of WSSV, AMO-miR-1000, wsv191-siRNA, and/or wsv407-siRNA, shrimp hemocytes were analyzed with Western blot analysis to detect the wsv191 and wsv407 proteins. (C) Influence of miR-1000 silencing, wsv191 silencing or/and wsv407 silencing on WSSV infection in shrimp. The WSSV copy number of shrimp with different treatments was evaluated using quantitative real-time PCR. Numbers indicated the time points post-infection (**p < 0.01). (D) Cumulative mortality of shrimp. The mortality of shrimp was examined daily (*p < 0.05; **p < 0.01).