Figure 3.

N1 and R848 synergistically induce functional maturation of human MoDCs. (A–E) Human MoDCs cultured in the presence or absence of N1 (250 ng/ml) and/or R848 (250 ng/ml) for 48 h were incubated in triplicate with allogenic human peripheral blood CD4+ T lymphocytes (L) (10⁵ cells/well). (A) On day 4, 1 μCi of [³H]TdR was added to each well to pulse the cultures for another 18 h. The proliferation was measured as the average (mean ± SD) of [³H]TdR incorporation (CPM) of triplicate wells. (B) IFN-γ production by CD4+ T cells co-cultured with DCs (DCs:L = 1:250) for 3 days (mean ± SD, n = 3). (C–E) The expression of T-bet and Gata-3 by CD4+ T cells co-cultured with DCs (DCs:L = 1:250) for 3 days were measured by flow cytometry. (C) Representative dot plots. (D,E) The average (mean ± SD) of triplicate wells. Shown is the results of one representative of three independent experiments obtained from different donors. **p<0.01 according to one-way ANOVA followed by Tukey's post hoc test.