Figure 6.
N1 and R848 synergistically induce phenotypic maturation of mouse BMDCs. (A,C) Mouse BMDCs were incubated at 5 × 105 cells/ml in the absence (sham) or presence of N1 (125, 250 ng/ml) and R848 (125, 250 ng/ml) for 48 h before they were immuno-stained and analyzed by flow cytometry for the expression of the indicated surface molecules [inscribed numbers are the average (geometric mean fluorescence intensity ± SD) of three batches of male mice; gray = sham-treated]. (B,D) Cytokine levels in the culture supernatants for 48 h were quantitated by cytokine array (mean ± SD; n = 3). Shown are the results of one experiment representative of three from three different batches of male mice (n = 6). *p<0.05, **p<0.01, and ****p<0.0001 according to one-way ANOVA followed by Tukey's post hoc test.