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HT suppresses the LPS-induced phosphorylation of NF-κB and iNOS in RAW264.7 cells. (A-C) RAW264.7 cells were stimulated by LPS (0.25 µg/ml) and cultured with HT (0–12.5 µg/ml) for 15 min (A, B) or 6 hr (C). The levels of NF-κB and pNF-κB (A), ERK1/2 and pERK1/2 (B), and iNOS (C) were analyzed by western blotting. β-Actin was an internal control. Representative results of duplicate experiments are shown.
