Table 1. Prime sets (QuantiTect Primer Assay, QIAGEN) used in real-time RT-PCR.
Gene name | Assay name |
---|---|
iNOS (Nitric oxide synthase 2, inducible) | Mm_LOC673161_1_SG |
COX-2 (Prostaglandin-endoperoxide synthase 2) | Mm_Ptgs2_1_SG |
TNF-α (Tumor necrosis factor alpha) | Mm_Tnf_1_SG |
NF-κB (Nuclear factor of kappa light polypeptide gene enhancer in B-cells) | Mm_Nfkb1_1_SG |
IL-1β (Interleukin 1 beta) | Mm_Il1b_2_SG |
IL-6 (Interleukin 6) | Mm_Il6_1_SG |
IL-10 (Interleukin 10) | Mm_Il10_1_SG |
Gapdh (glyceraldehyde-3-phosphate dehydrogenase) | Mm_Gapdh_3_SG |