Figure 4.

Characterization of the σ₁₉-oscillator. (a) Contour plot showing the model-predicted oscillation amplitudes mapped on to the [P₇₀-σ_19,P₁₉-C1-ssra] phase space for a given refresh rate (0.026 min^–1). (b) (i–vi) Experimentally obtained deGFP time courses for the σ₁₉-oscillator exhibiting oscillatory and non-oscillatory behavior for various values of [P₇₀-σ₁₉, P₁₉-C1-ssra]. All points have been mapped on to the contour plot (a). P₇₀-deGFP template concentration and refresh rate were fixed at 8 nM and 0.026 min^–1, respectively, in all experiments.