Table 3.
Specificity of Alexandrium qPCR Assays.
| Species | Assays |
A. pacificum |
A. australiense |
A. catenella |
A. ostenfeldii |
A. minutum |
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| CAWD44 | CS300 | ATCJ33 | AT-YC-H | TRIA-E | AT-STH-M | CAWD135 | CAWD12 | CS324 | |||
| A. catenella | ACT-US-408-F/ACT-US-645-R | – | – | – | – | + | + | – | – | – | |
| US-412-F/US-642-R | – | – | – | – | + | + | – | – | – | ||
| AlexLSUf2/Alexgp1RevAF1 | – | – | – | – | + | + | – | – | – | ||
| A. pacificum | catF/catR | + | + | + | – | – | – | – | – | – | |
| ACTA-416-F/ACTA-605-R | + | + | – | – | – | – | – | – | – | ||
| A. australiense | AusTv2-F/AusTv2-R | – | – | + | + | – | – | – | – | – | |
| A. ostenfeldii | AO352-F/AO493-R | – | – | – | – | – | – | + | – | – | |
+, Target sequence amplified; -, target sequence not amplified.