FIGURE 7.

Effect of MenG depletion on aerobic respiration and intracellular ATP level. (A) Oxygen consumption by M. smegmatis MenG depletion strain after 72-h with and without treatment with ATC. The decolorization of methylene blue in the media was used as an indication of the oxygen consumption, taking the A₆₆₅ of methylene blue immediately after the addition of ATC (at 0 min) as 100%. (B) ATP accumulation over a 24-h period during the third sub-culturing from the 48-h to the 72-h time point with and without ATC. The averages of biological triplicates are shown with standard deviations. ATC, anhydrotetracycline.