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. 2018 Dec 18;9:3119. doi: 10.3389/fmicb.2018.03119

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Copyright © 2018 Rosario, Santiago, Mesri and Verdun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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vIL-6 increases phosphorylation of STAT3 and AID expression in B cells stimulated for CSR. (A) A representative experiment quantifying CSR to IgA after 24 h of CIT stimulation (+CIT) in CH12F3-2 cells transduced with the pSin plasmid (control), and KSHV genes (vFLIP, vGPCR, and vIL-6). Values on the graph show the percentage of IgA⁺ cells (% IgA) relative to the control. Below, (A) representative FACS plot displaying the percentage of CH12F3-2 cells that switched to IgA (inside red rectangle). (B) Left, Western blot images of total extract from CH12F3-2 cells transduced with the pSin plasmid (Empty) or with KSHV vIL-6 (vIL-6) and stimulated for 24 h without (–) or with (+) αCD40, IL-4, and TGFβ to induce CSR to IgA. Right, Graphs showing quantification in arbitrary units of phospho-STAT3 relative to total STAT3 (pSTAT3/STAT3), phospho-STAT6 relative to total STAT6 (pSTAT6/STAT6) and AID relative to Actin (AID/Actin).