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. 2018 Dec 18;8:632. doi: 10.3389/fonc.2018.00632

Figure 3.

Figure 3

NF-κB is dispensable for PD-L1+ ABC-DLBCL cells generation mediated by MALT1 protease activity. (A) ABC-DLBCL cells and BJAB cells were pretreated with VRPR (+) or not (-) for 12 h prior to exposure to CFSE-labeled Vγ9Vδ2 T lymphocytes (E) or not for 6 h. Bcl-xl, cleavage products of RelB or Bcl-10 were detected in sorted DLBCL cells by western blotting. β-Actin was used as the loading control. One representative experiment of three is depicted. (B) qRT-PCR analysis of relative IL-6, IL-10, and Bcl-xl mRNA levels in ABC-DLBCL cells and BJAB cells from (A). (C) ABC-DLBCL and BJAB cells were pretreated with QNZ (+) or not (–) for 12 h before being exposed to CFSE-labeled Vγ9Vδ2 T lymphocytes for 6 h. Bcl-xl was detected in sorted DLBCL cells by western blotting. β-Actin was used as the loading control. One representative experiment of three is depicted. (D) qRT-PCR analysis of relative IL-6, IL-10, and Bcl-xl mRNA levels in ABC-DLBCL cells and BJAB cells from (C). (E) Cytotoxicity of Vγ9Vδ2 T lymphocytes toward ABC-DLBCL cells or BJAB cells from (C) (n = 3). (F) Proportions of PD-L1+ ABC-DLBCL cells or BJAB cells from (C) (n = 3). Data are shown as the means ± SD, *p < 0.05; **p < 0.01.