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. 2018 Dec 18;8:632. doi: 10.3389/fonc.2018.00632

Figure 6.

Figure 6

Glutaminolysis enhanced PD-L1+ ABC-DLBCL cell generation to subvert the cytotoxicity of human Vγ9Vδ2 T lymphocytes. (A) Relative glutamate levels in DLBCL cells pretreated with vehicle or BPTES for 12 h. (B) DLBCL cells were pretreated with vehicle or BPTES for 12 h before being exposed to CFSE-labeled Vγ9Vδ2 T lymphocytes for 6 h. Proportions of PD-L1+ DLBCL cells were detected (n = 5). (C) Cytotoxicity of Vγ9Vδ2 T lymphocytes toward DLBCL cells from (B) (n = 5). (D) Relative glutamate levels in DLBCL cells pretreated with vehicle, anti-PD-L1 antibody, or anti-PD-L1 antibody + BPTES (α-PD-L1+B) for 12 h. (E) Cytotoxicity of Vγ9Vδ2 T lymphocytes toward ABC-DLBCL cells from (D) (n = 5). (F) Cytotoxicity of Vγ9Vδ2 T lymphocytes toward ABC-DLBCL cells pretreated with vehicle, z-VRPR-fmk, BPTES, or z-VRPR-fmk + BPTES for 12 h (n = 5). Data are shown as the means ± SD, *p < 0.05; **p < 0.01; ***p < 0.001.