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. 2018 Dec 18;9:1864. doi: 10.3389/fpls.2018.01864

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Copyright © 2018 Melo, Fraga, Silva, Ferreira, Brustolini, Carpinetti, Machado, Reis and Fontes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression patterns of new GmNACs during multiple stress. (A) The identity of the selected GmNAC genes. (B) Heatmap of the stress-induced expression of 8 new putative GmNAC genes, GmNAC065 and GmNAC85. For the expression analysis of randomly selected GmNACs, soybean seedlings were stressed with PEG (10%), tunicamycin (5 μg/mL) and salicylic acid (5 mM) for 0.5, 4 and 12 h (24 h, exclusively for PEG treatment) and the transcript accumulation was monitored by qRT-PCR. The UNK-2 gene was used as endogenous control, and gene-associated stress markers were used to monitor the effectiveness of treatment (on the left). The data presented in the figure comprise the qRT-PCR from Supplementary Figures 4, 5 and Supplementary Table 6.